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Biosynthesis of Raffinose and Stachyose from Sucrose via an Multienzyme System.

Appl. Environ. Microbiol.. 2019; 
TianChaoyu,YangJiangang,ZengYan,ZhangTong,ZhouYingbiao,MenYan,YouChun,ZhuYueming,SunYua
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Bacterial Expression System and STS from Arabidopsis thaliana were codon optimized according to usage bias in E. coli (see the gene sequences presented in the supplemental material) and synthesized by GenScript (Nanjing, China). Get A Quote

摘要

Herein, we present a biocatalytic method to produce raffinose and stachyose using sucrose as the substrate. An multienzyme system was developed using five enzymes, namely, sucrose synthase (SUS), UDP-glucose 4-epimerase (GalE), galactinol synthase (GS), raffinose synthase (RS), and stachyose synthase (STS), and two intermedia, namely, UDP and inositol, which can be recycled. This reaction system produced 11.1 mM raffinose using purified enzymes under optimal reaction conditions and substrate concentrations. Thereafter, a stepwise cascade reaction strategy was employed to circumvent the instability of RS and STS in this system, and a 4.2-fold increase in raffinose production was obse... More

关键词

in vitro metabolic engineering,multienzyme system,soybean oligosaccharides,stepwise cascade reaction,suc