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Sensitive detection of glucagon aggregation using amyloid fibril-specific antibodies.

Biotechnol. Bioeng.. 2019; 
StimpleSamuel D,KalyoncuSibel,DesaiAlec A,MogensenJesper E,SpangLotte T,AsgreenDésirée J,StabyArne,TessierPet
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Biochemicals … from Acros (Geel, Belgium). Synthetic IAPP 20–29 was purchased from the WM Keck Foundation Biotechnology Resource Laboratory (Yale University) and GenScript Corp. (Piscataway, NJ) at >98% purity. The stock was dissolved … Get A Quote

摘要

Sensitive detection of protein aggregates is important for evaluating the quality of biopharmaceuticals and detecting misfolded proteins in several neurodegenerative diseases. However, it is challenging to detect extremely low concentrations (<10 ppm) of aggregated protein in the presence of high concentrations of soluble protein. Glucagon, a peptide hormone used in the treatment of extreme hypoglycemia, is aggregation-prone and forms amyloid fibrils. Detection of glucagon fibrils using conformation-specific antibodies is an attractive approach for identifying such aggregates during process and formulation development. Therefore, we have used yeast surface display and magnetic-activated cell sorting... More

关键词

aggregate,antibody,conformation,directed evolution,pro