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Knockdown of porcine endogenous retroviruses by RNA interference in Chinese experimental miniature pig fibroblasts.

Transplant. Proc.. 2013; 
LiZ-G,LiuG-B,PanM-X,WuQ-S,GeM,DuJ,WangY,
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Custom RNAi Services … Using the online RNAi software GenScript siRNA Target Finder (http://www.genscript.com/ siRNA_target_finder.html) and BLOCK-iT RNAi Designer (https://rnaidesigner.invitrogen.com … Sample total RNA was extracted using an RNA Extraction kit (Promega, Wis, United States) … Get A Quote

摘要

The clinical application of porcine-derived xenotransplants is limited by the potential risk of infection due to the presence of porcine endogenous retrovirus (PERV) in tissues, organs, and cells. The establishment of pig fibroblasts with low PERV expression and without PERV-C can provide a nuclear donor to generate a safer transgenic pig.

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