至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Specific cancer-associated mutations in the switch III region of Ras increase tumorigenicity by nanocluster augmentation.

Elife. 2015; 
ŠolmanMaja,LigabueAlessio,BlaževitšOlga,JaiswalAlok,ZhouYong,LiangHong,LectezBenoit,KopraKari,GuzmánCamilo,HärmäHarri,HancockJohn F,AittokallioTero,AbankwaDa
Products/Services Used Details Operation
Mutagenesis Services Site directed mutagenesis was done by GenScript USA Inc. (Piscataway, NJ, 468 USA) to introduce the specific mutations. Get A Quote

摘要

Hotspot mutations of Ras drive cell transformation and tumorigenesis. Less frequent mutations in Ras are poorly characterized for their oncogenic potential. Yet insight into their mechanism of action may point to novel opportunities to target Ras. Here, we show that several cancer-associated mutations in the switch III region moderately increase Ras activity in all isoforms. Mutants are biochemically inconspicuous, while their clustering into nanoscale signaling complexes on the plasma membrane, termed nanocluster, is augmented. Nanoclustering dictates downstream effector recruitment, MAPK-activity, and tumorigenic cell proliferation. Our results describe an unprecedented mechanism of signaling prot... More

关键词

Ras,biophysics,cancer,cell biology,human,membrane,nanoclustering,signalling,structural bio