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The functions of the variable lipoprotein family of Mycoplasma hyorhinis in adherence to host cells.

Vet. Microbiol.. 2016; 
XiongQiyan,WangJia,JiYan,NiBo,ZhangBixiong,MaQinghong,WeiYanna,XiaoShaobo,FengZhixin,LiuMaojun,ShaoGuo
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Monoclonal Antibody Services The adherence was evaluated by adding 100ml of mouse antiTrx-tag monoclonal antibody (1:200) (GenScript, Nanjing, China), followed by 100ml of FITC-conjugated goat anti-mouse IgG (1:200). Get A Quote

摘要

Mycoplasma hyorhinis (M. hyorhinis) is a swine pathogen that is associated with various human cancers and contamination in cell cultures. However, no studies on the adhesion molecules of this pathogen have yet been reported. The variable lipoprotein (Vlp) family is an important surface component of M. hyorhinis. Herein, we performed several experiments to identify the function of the Vlp family in adherence to host cells. Seven recombinant Vlp (rVlp) proteins were expressed in Escherichia coli and purified by affinity chromatography. The potential role of rVlp adherence to pig kidney (PK-15) and swine tracheal epithelial (STEC) cells was then studied by indirect immunofluorescence assay and microtiter plate... More

关键词

Adhesion,Mycoplasma hyorhinis,Variable lipopro