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Impact of Cultivated Hosts on the Recombination of .

J. Virol.. 2019-04; 
OuedraogoRimnoma S,PitaJustin S,SomdaIrenée P,TraoreOumar,RoossinckMaril
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PCR Cloning and Subcloning … Alternatively, the entire genome of NV can be custom synthesized and cloned into a plasmid vector, pUC57 (GenScript USA Inc., 860 Centennial Avenue, Piscataway, NJ 08854). Upon cloning, the recombinant clones are verified by sequencing before taking for sub-cloning … Get A Quote

摘要

(CMV) is one of the most successful viruses known, infecting over 1,200 species of plants. Like other single-stranded RNA viruses, CMV is known to have a high potential for population diversity due to error-prone replication and short generation times. Recombination is also a mechanism that allows viruses to adapt to new hosts. Host genes have been identified that impact the recombination of RNA viruses by using single-cell yeast systems. To determine the impact that the natural plant host has on virus recombination, we used a high-recombination-frequency strain of CMV, LS-CMV, which belongs to subgroup II, in three different cultivated hosts: cv. Marengo (pepper), cv. Xanthi nc (tobacco), a... More

关键词

host impact,intermolecular recombination,intramolecular recombination,plant virus,virus evolu