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Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium.

Biotechnol Biofuels. 2019-01; 
RussoD A,ZedlerJ A Z,WittmannD N,MöllersB,SinghR K,BatthT S,van OortB,OlsenJ V,BjerrumM J,Jense
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PCR Cloning and Subcloning … The DNA sequence was custom-synthesized by GenScript (USA) and inserted into the RSF1010-based, self-replicating plasmid pDF-trc [34], containing a synthetic widely used trc promoter for gene expression, by standard restriction digest cloning (20 µL reaction), with the … Get A Quote

摘要

Cyanobacteria have the potential to become next-generation cell factories due to their ability to use CO, light and inorganic nutrients to produce a range of biomolecules of commercial interest. UTEX 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. To establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant AA10A, a lytic polysaccharide monooxygenase from the Gram-positive bacterium

关键词

Bacterial secretion,Cyanobacteria,General secretory pathway,Lytic polysaccharide monooxygenase,Synechococcus elongatus UTEX 2973,TfAA10A,Twin-arginine-transloca