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Biochemical characterization of RNA-guided ribonuclease activities for CRISPR-Cas9 systems

Methods.. 2019-06; 
Gramelspacher MJ, Hou Z, Zhang Y
Products/Services Used Details Operation
Custom DNA/RNA Oligos To determine if codon optimization is needed for optimal Cas9 or Acr expression in E. coli, the online tool “GenScript Rare Codon Analysis” can be used. Get A Quote

摘要

The majority of bacteria and archaea rely on CRISPR-Cas systems for RNA-guided, adaptive immunity against mobile genetic elements. The Cas9 family of type II CRISPR-associated DNA endonucleases generates programmable double strand breaks in the CRISPR-complementary DNA targets flanked by the PAM motif. Nowadays, CRISPR-Cas9 provides a set of powerful tools for precise genome manipulation in eukaryotes and prokaryotes. Recently, a few Cas9 orthologs have been reported to possess intrinsic CRISPR-guided, sequence-specific ribonuclease activities. These discoveries fundamentally expanded the targeting capability of CRISPR-Cas9 systems, and promise to provide new CRISPR tools to manipulate specific cellular RNA tra... More

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