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LC-MS/MS method for simultaneous quantification of heparan sulfate and dermatan sulfate in urine by butanolysis derivatization.

Clin. Chim. Acta. 2019-01; 
ForniGiulia,MalvagiaSabrina,FunghiniSilvia,ScolamieroEmanuela,MuraMassimo,Della BonaMaria,VillanelliFabio,DamianoRoberta,la MarcaGianc
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摘要

Mucopolysaccharidoses are a group of lysosomal storage disorders (LSDs) characterized by the accumulation of glycosaminoglycans (GAGs). Recently, LC-MS/MS has been widely applied in GAGs analysis combined with different sample preparations for cleaving GAGs to disaccharide units. The aim of the present is paper is to present a new method for the simultaneous quantification of urinary dermatan sulfate (DS) and heparan sulfate (HS) by LC-MS/MS, after butanolysis reaction. Chromatographic separation was achieved with a gradient of acetonitrile and water in 0.1% formic acid on a Kinetex Biphenyl analytical column in 21 min. Calibration curves ranging from 0.78 to 50 μg/mL for HS and from 1.56 to 100 μg/... More

关键词

Butanolysis derivatization,Dermatan sulfate,Glycosaminoglycans,Heparan sulfate,LC-MS/MS,Mucopolysaccharid