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Hydrogen sulfide upregulates renal AQP-2 protein expression and promotes urine concentration.

FASEB J.. 2019-01; 
LuoRenfei,HuShan,LiuQiaojuan,HanMengke,WangFeifei,QiuMiaojuan,LiSuchun,LiXiaosa,YangTianxin,FuXiaodong,WangWeidong,LiChun
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PCR and Cloning … The 3.8 kb Tscot homology arm was amplified using a long range PCR strategy (Invitrogen, Accuprime Pfx DNA polymerase), incorporating ClaI and FseI sites into the PCR primers, and cloned into the Genscript plasmid (pMups) asymmetrically using the ClaI and FseI sites … Get A Quote

摘要

Increasing evidence supports the important role of HS in renal physiology and the pathogenesis of kidney injury. Whether HS regulates water metabolism in the kidney and the potential mechanism are still unknown. The present study was conducted to determine the role of HS in urine concentration. Inhibition of both cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS), 2 major enzymes for endogenous HS production, with propargylglycine (PPG) and amino-oxyacetate (AOAA), respectively, caused increased urine output and reduced urine osmolality in mice that was associated with decreased expression of aquaporin (AQP)-2 in the renal inner medulla. Mice treated with both PPG and AOAA developed a urin... More

关键词

cAMP,gasotransmitter,kidney,water cha