Products/Services Used | Details | Operation |
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ORF cDNA Clones/MolecularCloud> | To generate H. pylori strains harboring an mCherry fluorescent reporter, a 734-bp DNA fragment containing the ribosome binding site of cagA followed by the ORF encoding mCherry, a variant of the Discosoma red (DsRed) protein (47), was synthesized (GenScript USA Inc., Piscataway, NJ). | Get A Quote |
CagA is a secreted effector protein that contributes to gastric carcinogenesis. Previous studies showed that there is variation among strains in the steady-state levels of CagA and that a strain-specific motif downstream of the transcriptional start site (the +59 motif) is associated with both high levels of CagA and premalignant gastric histology. The 5' untranslated region contains a predicted stem-loop-forming structure adjacent to the +59 motif. In the current study, we investigated the effect of the +59 motif and the adjacent stem-loop on transcript levels and mRNA stability. Using site-directed mutagenesis, we found that mutations predicted to disrupt the stem-loop structure resulted in decreased... More