| Products/Services Used | Details | Operation |
|---|---|---|
| Nucleic Acid Purification & Analysis | … 2.9. Western blot assay. Cells were lysed using RIPA buffer. The BCA method was used to detect the protein concentration. Then, 25 μg of protein was loaded on 4–20% ExpressPLUS TM PAGE gels (GenScript, USA) for separation and transferred onto PVDF membranes … | Get A Quote |
Accumulating reports suggest that long noncoding RNAs (lncRNAs) play critical roles in the progression of many tumors. In this study， we explored the expression level of lncRNA FOXD2-AS1 in the tumorigenesis of hepatocellular carcinoma (HCC). The data indicated that FOXD2-AS1 expression was increased in HCC specimens and cell lines. Furthermore， aberrant expression was correlated with tumor number and tumor size in HCC patients. Silencing FOXD2-AS1 arrest cell cycle in the G0/G1 phase， inhibited colony formation， cell proliferation and suppressed the in vivo growth of subcutaneous tumors. Our results revealed that FOXD2-AS1 could epigenetically silence CDKN1B by recruiting EZH2 to CDKN1B promoter region... More
