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Damage on plants activates Ca-dependent metacaspases for release of immunomodulatory peptides.

Science. 2019-03; 
HanderTim,Fernández-Fernándezálvaro D,KumpfRobert P,WillemsPatrick,SchatowitzHendrik,RombautDebbie,StaesAn,NolfJonah,PottieRobin,YaoPanfeng,Gon?alvesAmanda,PavieBenjamin,BollerThomas,GevaertKris,Van BreusegemFrank,BartelsSebastian,StaelS
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摘要

Physical damage to cells leads to the release of immunomodulatory peptides to elicit a wound defense response in the surrounding tissue. In , the plant elicitor peptide 1 (Pep1) is processed from its protein precursor, PRECURSOR OF PEP1 (PROPEP1). We demonstrate that upon damage, both at the tissue and single-cell levels, the cysteine protease METACASPASE4 (MC4) is instantly and spatiotemporally activated by binding high levels of Ca and is necessary and sufficient for Pep1 maturation. Cytosol-localized PROPEP1 and MC4 react only after loss of plasma membrane integrity and prolonged extracellular Ca entry. Our results reveal that a robust mechanism consisting of conserved molecular components links the ... More

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