CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity.

CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However， precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy， which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover， we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore， our findings highlight the high sensitivity and accuracy of CDetection， which provides an efficient and highly... More