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The lysine methyltransferase SMYD2 methylates the kinase domain of type II receptor BMPR2 and stimulates bone morphogenetic protein signaling.

J. Biol. Chem.. 2017; 
Gao Shuman,Wang Zhiqiang,Wang Wencai,Hu Xueli,Chen Peilin,Li Jiwen,Feng Xinhua,Wong Jiemin,Du Jam
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Molecular Biology Tools and phosphatase inhibitor cocktails (Biotool), Lipofectamine 2000 (Invitrogen), BMP2 (GenScript Get A Quote

摘要

Lysine methylation of chromosomal and nuclear proteins is a well-known mechanism of epigenetic regulation, but relatively little is known about the role of this protein modification in signal transduction. Using an RNAi-based functional screening of the SMYD family of lysine methyltransferases (KMTs), we identified SMYD2 as a KMT essential for robust bone morphogenic protein (BMP)- but not TGFβ-induced target gene expression in HaCaT keratinocyte cells. A role for SMYD2 in BMP-induced gene expression was confirmed by shRNA knockdown and CRISPR/Cas9-mediated knock-out of We further demonstrate that knockdown or knock-out impairs BMP-induced phosphorylation of the signal-transducing protein SMAD1/5 and SMA... More

关键词

SMAD transcription factor,protein methylation,receptor,signal transduction,transcription regula