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Chanzyme TRPM7 Mediates the Ca Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation.

Immunity. 2018; 
Schappe Michael S,Szteyn Kalina,Stremska Marta E,Mendu Suresh K,Downs Taylor K,Seegren Philip V,Mahoney Michelle A,Dixit Sumeet,Krupa Julia K,Stipes Eric J,Rogers Jason S,Adamson Samantha E,Leitinger Norbert,Desai Bim
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Nucleic Acid Purification & Analysis instructions. Samples were loaded into 4%–20% or 12% ExpressPlus PAGE gels (GenScript; #M42012 Get A Quote

摘要

Toll-like receptors (TLRs) sense pathogen-associated molecular patterns to activate the production of inflammatory mediators. TLR4 recognizes lipopolysaccharide (LPS) and drives the secretion of inflammatory cytokines, often contributing to sepsis. We report that transient receptor potential melastatin-like 7 (TRPM7), a non-selective but Ca-conducting ion channel, mediates the cytosolic Ca elevations essential for LPS-induced macrophage activation. LPS triggered TRPM7-dependent Ca elevations essential for TLR4 endocytosis and the subsequent activation of the transcription factor IRF3. In a parallel pathway, the Ca signaling initiated by TRPM7 was also essential for the nuclear translocation of NFκB. Co... More

关键词

LPS,TLR4,TRP channel,TRPM7,endotoxin,inflammation,ion channel,sepsis,toll-like rece