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The complete structure of the chloroplast 70S ribosome in complex with translation factor pY.

EMBO J.. 2017; 
Bieri Philipp,Leibundgut Marc,Saurer Martin,Boehringer Daniel,Ban N
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摘要

Chloroplasts are cellular organelles of plants and algae that are responsible for energy conversion and carbon fixation by the photosynthetic reaction. As a consequence of their endosymbiotic origin, they still contain their own genome and the machinery for protein biosynthesis. Here, we present the atomic structure of the chloroplast 70S ribosome prepared from spinach leaves and resolved by cryo-EM at 3.4?? resolution. The complete structure reveals the features of the 4.5S rRNA, which probably evolved by the fragmentation of the 23S rRNA, and all five plastid-specific ribosomal proteins. These proteins, required for proper assembly and function of the chloroplast translation machinery, bind and st... More

关键词

chloroplast,cryo‐EM,pY,ribosome,transla