| Products/Services Used | Details | Operation |
|---|---|---|
| Mammalian Expression System | HC (gamma 1) and LC (kappa) cDNAs were commercially synthesized (Genscript) and cloned into separate expression plasmid vectors under the control of the same human cytomegalovirus (CMV) immediate-early enhancer and promoter. | Get A Quote |
As the demand for monoclonal antibodies (mAb) increases， more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cell lines which leads to expression reduction and instability. There are reports on improved antibody expression in ubiquitous chromatin opening element (UCOE) containing both heavy and light chain gene constructs. Here we investigate the impact of having these elements as part of the light chain， heavy chain or both genes during cell line development. In this regard， non-UCOE and UCOE vectors were constructed and stable Chinese hamster ovary (CHO) cell pools were generated by different vector combinat... More
