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Production of 3-geranyl-4-hydroxybenzoate acid in yeast, an important intermediate of shikonin biosynthesis pathway.

FEMS Yeast Res.. 2017; 
Wang Sheng,Wang Ruishan,Liu Tan,Zhan Zhilai,Kang Liping,Wang Yanan,Lv Chaogeng,Werck-Reichhart Daniele,Guo Lanping,Huang
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摘要

Shikonin and its derivatives are the main active components in the medicinal plant Arnebia euchroma and possess extensive pharmaceutical properties. In this study, we developed an optimized yeast system to obtain high-level production of 3-geranyl-4-hydroxybenzoate acid (GBA), an important intermediate involved in shikonin biosynthesis pathway. For host selection, recombinant expression of p-hydroxybenzoate:geranyltransferase (PGT) derived from A. euchroma was performed in Saccharomyces cerevisiae WAT11U strain and high yield monoterpene strain. In shake flask culture with 1 mM p-hydroxybenzoate acid (PHBA), they could yield GBA at 0.1567 and 20.8624 mg L-1, respectively. Additionally, AePGT6 showed... More

关键词

3-geranyl-4-hydroxybenzoate acid,biosynthesis,biotransformation,shikonin,y