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High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow.

Stem Cell Res Ther. 2018;

Watanabe Ken，Otabe Koji，Shimizu Norio，Komori Keiichirou，Mizuno Mitsuru，Katano Hisako，Koga Hideyuki，Sekiya Ic

Products/Services Used	Details	Operation
PCR and Cloning	The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2, and 1 mM dNTPs (genscript).	Get A Quote

摘要

Latent microorganism infection is a safety concern for the clinical application of mesenchymal stem cells (MSCs). The aim of this study is to investigate the frequencies and sensitivities of the latent virus and mycoplasma infections in synovium， bone marrow， peripheral blood cells， and blood plasma and cultured synovial MSCs.