| Products/Services Used | Details | Operation |
|---|---|---|
| PCR and Cloning | Ten (10) µl of the material were used in a 100 µl PCR reaction including 10x Taq buffer, 800nM BG4APCR primer (Table S1), dNTPs (0.2 mM), 2U Taq DNA polymerase (GenScript) and water to volume. | Get A Quote |
Peach rosette mosaic disease was first described in the 1940s affecting peach and plum. It was later determined that peach rosette mosaic virus (PRMV) is the causal agent of the disease. PRMV， a member of the genus Nepovirus， infects several perennial crops including stone fruit， grape and blueberry as well as several weed species found in orchards around the world. The molecular characterization of the virus is limited to partial genome sequences making it difficult to develop reliable and sensitive molecular detection tests; the reason that detection is routinely performed using ELISA with antibodies risen against a single virus isolate. Given the potential economic impact of the virus and the modes of ... More
