至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Divergent T-cell receptor recognition modes of a HLA-I restricted extended tumour-associated peptide.

Nat Commun. 2018; 
Chan Kok Fei,Gully Benjamin S,Gras Stephanie,Beringer Dennis X,Kjer-Nielsen Lars,Cebon Jonathan,McCluskey James,Chen Weisan,Rossjohn J
Products/Services Used Details Operation
Nucleic Acid Purification & Analysis Five full-length genes encoding TCR α- and β-chains (Table 1) separated by a hydrolase element P2A linker58 were synthesised (GenScript) and subcloned into the pMIG expression vector58. Get A Quote

摘要

Human leukocyte antigen (HLA)-I molecules generally bind short peptides (8-10 amino acids), although extended HLA-I restricted peptides (>10 amino acids) can be presented to T cells. However, the function of such extended HLA-I epitopes in tumour immunity, and how they would be recognised by T-cell receptors (TCR) remains unclear. Here we show that the structures of two distinct TCRs (TRAV4TRAJ21-TRBV28TRBJ2-3 and TRAV4 TRAJ8-TRBV9TRBJ2-1), originating from a polyclonal T-cell repertoire, bind to HLA-B*07:02, presenting a 13-amino-acid-long tumour-associated peptide, NY-ESO-1. Comparison of the structures reveals that the two TCRs differentially binds NY-ESO-1-HLA-B*07:02 complex, and induces d... More

关键词