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Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration.

Plant J.. 2017; 
Lynch Joseph H,Orlova Irina,Zhao Chengsong,Guo Longyun,Jaini Rohit,Maeda Hiroshi,Akhtar Tariq,Cruz-Lebron Junellie,Rhodes David,Morgan John,Pilot Guillaume,Pichersky Eran,Dudareva Nat
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Nucleic Acid Purification & Analysis The 1223 bp synthetic cDNA containing PAL isoforms specific regions described above was introduced into a modified pUC57 vector (pUC57-simple, Genscript, NJ) to create pUC57ConA. Get A Quote

摘要

Detrimental effects of hyperaccumulation of the aromatic amino acid phenylalanine (Phe) in animals, known as phenylketonuria, are mitigated by excretion of Phe derivatives; however, how plants endure Phe accumulating conditions in the absence of an excretion system is currently unknown. To achieve Phe hyperaccumulation in a plant system, we simultaneously decreased in petunia flowers expression of all three Phe ammonia lyase (PAL) isoforms that catalyze the non-oxidative deamination of Phe to trans-cinnamic acid, the committed step for the major pathway of Phe metabolism. A total decrease in PAL activity by 81-94% led to an 18-fold expansion of the internal Phe pool. Phe accumulation had multifaceted ... More

关键词

Petunia hybrida ,aromatic amino acids,phenylalanine,phenylalanine ammonia lyase,phenylpropanoids,regulation,shikimate pat