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Dendritic Cell Targeting Effectively Boosts T Cell Responses Elicited by an HIV Multiepitope DNA Vaccine.

Front Immunol. 2017; 
Apostólico Juliana de Souza,Lunardelli Victória Alves Santos,Yamamoto Marcio Massao,Souza Higo Fernando Santos,Cunha-Neto Edecio,Boscardin Silvia Beatriz,Rosa Daniela San
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Nucleic Acid Purification & Analysis The artificial gene (Genscript) was cloned between the HindIII and XhoI restriction sites of the pVAX1 vector (Invitrogen) to generate the pVAXHIVBr8 plasmid that was amplified using DH5α cells. Get A Quote

摘要

Despite several efforts in the last decades, an efficacious HIV-1 vaccine is still not available. Different approaches have been evaluated, such as recombinant proteins, viral vectors, DNA vaccines, and, most recently, dendritic cell (DC) targeting. This strategy is based on DC features that place them as central for induction of immunity. Targeting is accomplished by the use of chimeric monoclonal antibodies directed to DC surface receptors fused to the antigen of interest. In this work, we targeted eight promiscuous HIV-derived CD4 T cell epitopes (HIVBr8) to the DEC205 DCs by fusing the multiepitope immunogen to the heavy chain of αDEC205 (αDECHIVBr8), in the presence of the TLR3 agonist ... More

关键词

CD4+ T cell,HIV,dendritic cells,monoclonal antibody,multiepitope vac