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Nucleic Acid Purification & Analysis> | A shRNA target to ARID1A (1: 5’-GCCAAGGAGAGCAGAGTAA-3’, 2: 5’-GCCCTAACATGGCCAATAT-3’) and a control scramble sequence (shCon) (5’-GATCCCCTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTT-3’) were synthesized by and purchased from Genscript (Nanjing, China) and inserted into the BamHI/ HindIII site of linearized pRNA-H1.1 vector to yield the pRNA-H1. | Get A Quote |
Neuroblastoma is the most common extracranial solid tumor in childhood which often acquires drug resistance and becomes aggressive phenotypes. The high-risk patients suffer from high mortality due to the limitation of the treatment strategies. ARID1A (AT-rich interactive domain-containing protein 1A), a subunit of SWI/SNF complexes, is considered as a tumor suppressor in many cancers. The aim of the present study was to investigate the effect of ARID1A on migration and invasion in neuroblastoma cells. The shRNA targeting ARID1A was designed and delivered into SK-N-SH cells to knock down ARID1A expression. Knockdown of ARID1A by shRNA significantly increased the viability and invasion ability, and caused G... More