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Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex.

Cell Rep. 2019; 
Tian Songhai,Wu Quan,Zhou Bo,Choi Mei Yuk,Ding Bo,Yang Wei,Dong
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摘要

The endoplasmic reticulum (ER) membrane protein complex (EMC) is a key contributor to biogenesis and membrane integration of transmembrane proteins, but our understanding of its mechanisms and the range of EMC-dependent proteins remains incomplete. Here, we carried out an unbiased mass spectrometry (MS)-based quantitative proteomic analysis comparing membrane proteins in EMC-deficient cells to wild-type (WT) cells and identified 36 EMC-dependent membrane proteins and 171 EMC-independent membrane proteins. Of these, six EMC-dependent and six EMC-independent proteins were further independently validated. We found that a common feature among EMC-dependent proteins is that they contain transmembrane domains (... More

关键词

EMC,ER membrane protein complex,charged residue,ion channels,mass spectrometry-based proteomics,membrane protein synthesis,polar residue,transmembrane domain,transporter,transporter acti