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Global phosphoproteomic analysis of Ebola virions reveals a novel role for VP35 phosphorylation-dependent regulation of genome transcription.

Cell Mol Life Sci. 2019; 
Ivanov Andrey,Ramanathan Palaniappan,Parry Christian,Ilinykh Philipp A,Lin Xionghao,Petukhov Michael,Obukhov Yuri,Ammosova Tatiana,Amarasinghe Gaya K,Bukreyev Alexander,Nekhai Se
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Nucleic Acid Purification & Analysis The sequences of WT VP35 and mutants with C-terminal Flag sequence were synthesized by GenScript and cloned in pcDNA3.1(−) vector at NotI and KpnI sites Get A Quote

摘要

Ebola virus (EBOV) causes severe human disease with a high case fatality rate. The balance of evidence implies that the virus circulates in bats. The molecular basis for host-viral interactions, including the role for phosphorylation during infections, is largely undescribed. To address this, and to better understand the biology of EBOV, the phosphorylation of EBOV proteins was analyzed in virions purified from infected monkey Vero-E6 cells and bat EpoNi/22.1 cells using high-resolution mass spectrometry. All EBOV structural proteins were detected with high coverage, along with phosphopeptides. Phosphorylation sites were identified in all viral structural proteins. Comparison of EBOV protein phosphory... More

关键词

Ebola virus,Phosphorylation,Replication,Transcrip