| Products/Services Used | Details | Operation |
|---|---|---|
| Nucleic Acid Purification & Analysis | Tail DNA was extracted using the GenScript TissueDirect Multiplex PCR System (GenScript Corporation, Piscataway, NJ, USA) and subjected to PCR using a bufer containing 1.0 U/50μL Taq DNA polymerase, 45mM KCl, 2.5mM Mg2+, 200μM dNTP (Eppendorf Hotmastermix, Eppendorf AG, Hamburg, Germany) and 0.4μM of the corresponding primers (forward primers for WT and St8sia2−/− were 5′-cctctctcgtgtacccactgccat-3′ and 5′-aggctccctcactgctgtcta-3′, respectively, and the shared reverse primer was 5′-gggaacagcgctcataagat-3′). | Get A Quote |
ST8 alpha-N-acetyl-neuraminide alpha-2，8-sialyltransferase 2 (ST8SIA2) synthesizes polysialic acid (PSA)， which is essential for brain development. Although previous studies reported that St8sia2-deficient mice that have a mixed 129 and C57BL/6 (B6) genetic background showed mild and variable phenotypes， the reasons for this remain unknown. We hypothesized that this phenotypic difference is caused by diversity in the expression or function of flanking genes of St8sia2. A genomic polymorphism and gene expression analysis in the flanking region revealed reduced expression of insulin-like growth factor 1 receptor (Igf1r) on the B6 background than on that of the 129 strain. This observation， along with the ... More
