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Experimental comparison of energy landscape features of ubiquitin family proteins.

Proteins. 2019; 
Nandi Tathagata,Yadav Anju,Ainavarapu Sri Rama
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摘要

Small ubiquitin-related modifiers (SUMO1 and SUMO2) are ubiquitin family proteins, structurally similar to ubiquitin, differing in terms of their amino acid sequence and functions. Therefore, they provide a great platform for investigating sequence-structure-stability-function relationship. Here, we used chemical denaturation in comparing the folding-unfolding pathways of the SUMO proteins with their structural homologue ubiquitin (UF45W-pseudo wild-type [WT] tryptophan variant) with structurally analogous tryptophan mutations (SUMO1 [S1F66W], SUMO2 [S2F62W]). Equilibrium denaturation studies report that ubiquitin is the most stable protein among the three. The observed denaturant-dependent folding ra... More

关键词

Chevron plots,SUMOs,chemical denaturation,energy landscape,intermediates,protein folding,ubiqu