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Structure of the Centromere Binding Factor 3 Complex from Kluyveromyces lactis.

J. Mol. Biol.. 2019; 
LeePhong D,WeiHui,TanDongyan,HarrisonSteph
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Bacterial Expression System lactis Ndc10 gene was codon optimized for bacterial expression (GenScript) and sub-cloned into a pET vector modified for ligation- independent cloning and encoding an N-terminal maltose binding protein tag and TEV protease cleavage site. Get A Quote

摘要

Kinetochores are the multiprotein complexes that link chromosomal centromeres to mitotic-spindle microtubules. Budding yeast centromeres comprise three sequential "centromere-determining elements", CDEI, II, and III. CDEI (8 bp) and CDEIII (~25 bp) are conserved between Kluyveromyces lactis and Saccharomyces cerevisiae, but CDEII in the former is twice as long (160 bp) as CDEII in the latter (80 bp). The CBF3 complex recognizes CDEIII and is required for assembly of a centromeric nucleosome, which in turn recruits other kinetochore components. To understand differences in centromeric nucleosome assembly between K. lactis and S. cerevisiae, we determined the structure of a K. lactis CBF3 complex by e... More

关键词

budding yeast,centromeric nucleosome assembly,electron cryomicroscopy,kinetochore,point centro