A taxonomic study of a Gram-stain negative， rod-shaped， motile， asporogenous， catalase- and oxidase-positive bacterium， sh-6， forming pink-red colonies， isolated from a contaminated R2A plate in the laboratory was performed. Its optimum growth temperature was determined to be 28?°C in the absence of NaCl on R2A plates. On the basis of 16S rRNA gene sequence analysis， strain sh-6 belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3 (95.05%)， Hymenobacter paludis KBP-30 (94.96%)， Hymenobacter coalescens WW84 (94.04%)， Hymenobacter gummosus ANT-18 (93.38%)， Hymenobacter ocellatus Myx2105 (93.70%)， Hymenobacter jeollabukensis 1-3-3-8 (93.48%) and Hymenoba... More
A taxonomic study of a Gram-stain negative， rod-shaped， motile， asporogenous， catalase- and oxidase-positive bacterium， sh-6， forming pink-red colonies， isolated from a contaminated R2A plate in the laboratory was performed. Its optimum growth temperature was determined to be 28?°C in the absence of NaCl on R2A plates. On the basis of 16S rRNA gene sequence analysis， strain sh-6 belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3 (95.05%)， Hymenobacter paludis KBP-30 (94.96%)， Hymenobacter coalescens WW84 (94.04%)， Hymenobacter gummosus ANT-18 (93.38%)， Hymenobacter ocellatus Myx2105 (93.70%)， Hymenobacter jeollabukensis 1-3-3-8 (93.48%) and Hymenobacter koreensis GYR3077 (93.21%). Comparison of the genome of strain sh-6 and that of H. gummosus ANT-18 gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%， respectively. The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine， respectively. The major cellular fatty acids identified as iso-C， summed feature 4 (iso-C I/anteiso B)， iso-C， iso-C 3-OH and iso-C. The major polar lipid of strain sh-6 determined to be phosphatidylethanolamine. The DNA G+C content was determined to be 60.5?mol%. On the basis of the evidence presented in this study， a novel species of the genus Hymenobacter， Hymenobacter oligotrophus sp. nov.， is proposed， with the type strain sh-6 (=?CCTCC AB 2016064?=?KCTC 62345).
