A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed. Its optimum growth temperature was determined to be 28?°C in the absence of NaCl on R2A plates. On the basis of 16S rRNA gene sequence analysis, strain sh-6 belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3 (95.05%), Hymenobacter paludis KBP-30 (94.96%), Hymenobacter coalescens WW84 (94.04%), Hymenobacter gummosus ANT-18 (93.38%), Hymenobacter ocellatus Myx2105 (93.70%), Hymenobacter jeollabukensis 1-3-3-8 (93.48%) and Hymenoba... More
A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed. Its optimum growth temperature was determined to be 28?°C in the absence of NaCl on R2A plates. On the basis of 16S rRNA gene sequence analysis, strain sh-6 belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3 (95.05%), Hymenobacter paludis KBP-30 (94.96%), Hymenobacter coalescens WW84 (94.04%), Hymenobacter gummosus ANT-18 (93.38%), Hymenobacter ocellatus Myx2105 (93.70%), Hymenobacter jeollabukensis 1-3-3-8 (93.48%) and Hymenobacter koreensis GYR3077 (93.21%). Comparison of the genome of strain sh-6 and that of H. gummosus ANT-18 gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively. The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively. The major cellular fatty acids identified as iso-C, summed feature 4 (iso-C I/anteiso B), iso-C, iso-C 3-OH and iso-C. The major polar lipid of strain sh-6 determined to be phosphatidylethanolamine. The DNA G+C content was determined to be 60.5?mol%. On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp. nov., is proposed, with the type strain sh-6 (=?CCTCC AB 2016064?=?KCTC 62345).