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In vivo Ca imaging of astrocytic microdomains reveals a critical role of the amyloid precursor protein for mitochondria.

Glia. 2019; 
MontagnaElena,CruxSophie,LucknerManja,HerberJulia,ColomboAlessio V,Marinkovi?Petar,TahirovicSabina,LichtenthalerStefan F,WannerGerhard,MüllerUlrike C,SgobioCarmelo,HermsJo
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Proteins, Expression, Isolation and Analysis An amount of 10–15 μg protein was loaded on a gradient gel (M42015, Genscript ExpressPlus PAGE gel 4%–20%) and SDS-PAGE was performed with Tris-MOPS-SDS Buffer (M00138, Genscript). Get A Quote

摘要

The investigation of amyloid precursor protein (APP) has been mainly confined to its neuronal functions, whereas very little is known about its physiological role in astrocytes. Astrocytes exhibit a particular morphology with slender extensions protruding from somata and primary branches. Along these fine extensions, spontaneous calcium transients occur in spatially restricted microdomains. Within these microdomains mitochondria are responsible for local energy supply and Ca buffering. Using two-photon in vivo Ca imaging, we report a significant decrease in the density of active microdomains, frequency of spontaneous Ca transients and slower Ca kinetics in mice lacking APP. Mechanistically, these chan... More

关键词

in vivo Ca2+ imaging,amyloid precursor protein,astrocyte,microdomain,mitochondria fragmenta