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Rapid and Ultrasensitive Quantification of Multiplex Respiratory Tract Infection Pathogen via Lateral Flow Microarray based on SERS Nanotags.

Theranostics. 2019; 
ZhangDi,HuangLi,LiuBing,GeQinyu,DongJian,ZhaoXian
Products/Services Used Details Operation
Custom DNA/RNA Oligos All the nucleic acid oligo- nucleotides used in this study were purchased from GenScript (China) and the sequences are shown in Table 1. Get A Quote

摘要

Respiratory tract infections (RTIs) are severe acute infectious diseases, which require the timely and accurate identification of the pathogens involved so that the individual treatment plan can be selected, including optimized use of antibiotics. However, high throughput and ultrasensitive quantification of multiple nucleic acids is a challenge in a point of care testing (POCT) device. Herein, we developed a 2×3 microarray on a lateral flow strip with surface enhanced Raman scattering (SERS) nanotags encoding the nucleic acids of 11 common RTI pathogens. On account of the signal magnification of encoded SERS nanotags in addition to the high surface area to volume ratio of the nitrocellulose (NC) memb... More

关键词

core-shell SERS nanotags,lateral flow microarray,multiplex nucleic acids detection,respiratory tract infection (