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In situ imaging and interfering Dicer-mediated cleavage process via a versatile molecular beacon probe.

Anal. Chim. Acta. 2019; 
ZhangKai,YangXue-Jiao,ZhangTing-Ting,LiXiang-Ling,ChenHong-Yuan,XuJing-
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Custom DNA/RNA Oligos Oligonucleotides were purchased from Genscript Biotechnology Co. Get A Quote

摘要

A novel versatile locked nucleic acid modified molecular beacon probe (LNA-MB) was developed for imaging intracellular precursor miRNAs (pre-miRNAs) and disturbing Dicer-mediated cleavage process. The target recognition reaction between the smart probe and pre-miRNA can not only induce the conformational changes of probe and block the Dicer cleavage site, but also inhibit the cleavage process, and then achieve down-regulation of miRNA expression. Simultaneously, the target recognition reaction broke the fluorescence resonance energy transfer (FRET) between fluorophore donor FAM and acceptor TAMRA, which were labelled on the LNA-MB probe, further induced the relevant change of fluorescence signal, an... More

关键词

Dicer-mediated cleavage process,Imaging analysis,Locked nucleic acid,Regulation,micro