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Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against lung infection.

J. Biol. Chem.. 2019; 
LillehojErik P,GuangWei,HyunSang W,LiuAnguo,HegerleNicolas,SimonRaphael,CrossAlan S,IshidaHideharu,LuzinaIrina G,AtamasSergei P,GoldblumSime
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Bacterial Expression System 2) from PAK and PAO1, respectively, were codon optimized (GenScript) for expression in E. Get A Quote

摘要

(Pa) expresses an adhesin, flagellin, that engages the mucin 1 (MUC1) ectodomain (ED) expressed on airway epithelia, increasing association of MUC1-ED with neuraminidase 1 (NEU1) and MUC1-ED desialylation. The MUC1-ED desialylation unmasks both cryptic binding sites for Pa and a protease recognition site, permitting its proteolytic release as a hyperadhesive decoy receptor for Pa. We found here that intranasal administration of Pa strain K (PAK) to BALB/c mice increases MUC1-ED shedding into the bronchoalveolar compartment. MUC1-ED levels increased as early as 12 h, peaked at 24-48 h with a 7.8-fold increase, and decreased by 72 h. The a-type flagellin-expressing PAK strain and the b-type flagellin-... More

关键词

Pseudomonas aeruginosa (P. aeruginosa),adhesin,cell surface associated (MUC1),decoy protein,flagellin,mucin 1,neuraminidase,sialic acid,sialidase,virul