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Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157:H7.

Food Chem. 2019; 
WangChun,XingKeyu,ZhangGanggang,YuanMeifang,XuShaolan,LiuDaofeng,ChenWenyao,PengJuan,HuSong,LaiWei
Products/Services Used Details Operation
Custom DNA/RNA Oligos All oligonucleotides were synthesized by Genscript Co. Get A Quote

摘要

Escherichia coli O157:H7 (E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended block acted as a scaffold to prepare fluorescent Ag nanoclusters (AgNCs). The anchoring block contained Poly A, which bound with the surface of gold nanoparticles to quench the fluorescence of AgNCs. An interesting ELISA approach for detecting E. coli O157:H7 was established via fluorescent quenching of DNA-stabilized AgNCs by using a sandwich complex. The changes in fluorescence intensity of AgNCs were used to quantitatively detect E. coli O157:H7. The sensitivi... More

关键词

Ag nanoclusters,Escherichia coli O157:H7,Fluorescence quenching,Gold nanoparti