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AT-dinucleotide rich sequences drive fragile site formation.

Nucleic Acids Res.. 2019; 
Irony-Tur SinaiMichal,SalamonAnita,StanleighNoemie,GoldbergTchelet,WeissAryeh,WangYuh-Hwa,KeremBats
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DNA Sequencing Following verification of its length by gel electrophoresis, this PCR product was isolated and sequenced (Sanger Sequencing, GenScript USA Inc. Get A Quote

摘要

Common fragile sites (CFSs) are genomic regions prone to breakage under replication stress conditions recurrently rearranged in cancer. Many CFSs are enriched with AT-dinucleotide rich sequences (AT-DRSs) which have the potential to form stable secondary structures upon unwinding the double helix during DNA replication. These stable structures can potentially perturb DNA replication progression, leading to genomic instability. Using site-specific targeting system, we show that targeted integration of a 3.4 kb AT-DRS derived from the human CFS FRA16C into a chromosomally stable region within the human genome is able to drive fragile site formation under conditions of replication stress. Analysis of >1300 X c... More

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