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miRNA-30-3p improves myocardial ischemia via the PTEN/PI3K/AKT signaling pathway.

J. Cell. Biochem.. 2019; 
YinYugang,Yang
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Biochemicals 2 | Drugs and reagents High‐glucose Dulbecco’s modified Eagle’s medium (DMEM) (GibcoBRL, Brand Island, NY), fetal bovine serum (FBS) (GibcoBRL), Hoechst 33342 dye, propidium iodide (PI) dye (Shanghai Biyuntian Biological Technol- ogy Co, Ltd), benzylpenicillin sodium and streptomycin (North China Pharmaceutical Co, Ltd, China), trypsin (Hangzhou Hongbo Biological Engineering Co Ltd, Hangzhou, China), two dimethyl sulfoxide (Tianjin Yongda Chemical Reagent Co, Ltd, Tianjing, China), and miRNA‐30‐3p and PI3K inhibitor (Nanjing GenScript Co, Ltd, China). Get A Quote

摘要

The aim of this study was to explain the effect and mechanisms of miRNA-30-3p in myocardial ischemia-induced cell apoptosis in vitro and in?vivo studies. In the cell experiment, the H9C2 cells were?divided into the normal control (NC), and the model, miRNA, and miRNA?+?phosphatidylinositol 3-kinase (PI3K) inhibitor groups. The cell survival rates of the different?groups were measured with?the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay kit;?the lactate dehydrogenase (LDH), malondialdehyde (MDA) content, and?superoxidedimutase (SOD) activity in the bathing medium were?assayed for the evaluation of myocardial cell injury. The cell apoptosis rate of ... More

关键词

AKT,PTEN,cell apoptosis,miRNA-30-3p,myocardial ischemia,phosphatidylinositol 3-ki