| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | 6 Strategy for genetic engineering of NHase using bioinformatic tools Nitrile hydratase gene from soil/ Wastewater sample Optimization of codon using Genscript/ IDT Codon opƟmizaƟon tool Design of ribosome binding sequence by RBS Designer software/ Genome compiler Design of Spacer arm using Genscript tools Cloning, overexpression and purification of NHase Molecular Biology Reports DNA Extraction from microbial sources (Soil and water) Insertion of genes (depending on the insert size) into plasmid/ cosmid Metagenomic DNA library construction Sequence-based analysis Function-based analysis Picking up good NHase clones Biotechnology applications Fig. | Get A Quote |
Nitrile hydratase (NHase) is a prominent enzyme in many microorganisms for its nitrile metabolism. The potentiality in the bioconversion of nitriles to its high-value amides has been extensively used in industries for the production of acrylamide and nicotinamide which are essential chemicals. Enzymologists are still considering NHases for its potential biotechnological applications including biotransformations and bioremediations. But most of the nitrile hydratases have limitations like the low expression， low thermostability and enantioselectivity. Though considerable data has been generated in the area of gene configuration， crystal structure， kinetic mechanism and photoreactivity of NHases， there is... More
