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Ion- and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel.

BMC Biol.. 2017; 
OuXiaomin，GuoJianli，WangLongfei，YangHanting，LiuXiuying，SunJianyuan，LiuZhen
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PCR Cloning and Subcloning Methods Cloning, protein expression, and purification The gene encoding SsTRIC was synthesized (GenScript) with optimized codon usage for protein expression in Escherichia coli and inserted between the NdeI and XhoI sites in the pET21b vector.... After blocking and washing, the blot was probed by HRP-conjugated anti-Histag mAb antibody (GenScript, cat. Get A Quote

摘要

Trimeric intracellular cation (TRIC) channels are crucial for Ca handling in eukaryotes and are involved in K uptake in prokaryotes. Recent studies on the representative members of eukaryotic and prokaryotic TRIC channels demonstrated that they form homotrimeric units with the ion-conducting pores contained within each individual monomer.

关键词

Crystal structure，Gating mechanism，Ion channel，Membrane protein，Selecti
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