至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.

Nat. Med.. 2015; 
WangJing,SunChongxiu,GerdesNorbert,LiuConglin,LiaoMengyang,LiuJian,ShiMichael A,HeAina,ZhouYi,SukhovaGalina K,ChenHuimei,ChengXian Wu,KuzuyaMasafumi,MuroharaToyoaki,ZhangJie,ChengXiang,JiangMengmeng,ShullGary E,RogersShaunessy,YangChao-Ling,KeQiang,JelenSabina,BindelsRené,EllisonDavid H,JarolimPetr,LibbyPeter,ShiGuo-
Products/Services Used Details Operation
Mutagenesis Services 1 as a template, site- directed mutagenesis was performed to replace each of three residues located in the NH2-terminus of NCC that are sites of phosphorylation (Thr53, Thr58, and Ser71), or all three amino acids together, with alanine (GenScript USA Inc. Get A Quote

摘要

Interleukin-18 (IL18) participates in atherogenesis through several putative mechanisms. Interruption of IL18 action reduces atherosclerosis in mice. Here, we show that absence of the IL18 receptor (IL18r) does not affect atherosclerosis in apolipoprotein E-deficient (Apoe(-/-)) mice, nor does it affect IL18 cell surface binding to or signaling in endothelial cells. As identified initially by co-immunoprecipitation with IL18, we found that IL18 interacts with the Na-Cl co-transporter (NCC; also known as SLC12A3), a 12-transmembrane-domain ion transporter protein preferentially expressed in the kidney. NCC is expressed in atherosclerotic lesions, where it colocalizes with IL18r. In Apoe(-/-) mice, co... More

关键词