Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | We used the following antibodies in our studies: 8WG16 (GeneTex) at 1:1000, For immunoblotting, we followed standard procedures for SDS-PAGE and semi-dry 580 581 anti-Histone H3 (Genscript, Piscataway, NJ) at 1:1000, anti-TFIIIA (described above) at 1:400, anti- 582 FLAG L5 monoclonal antibody (Life Technologies) at 1:1000, HRP-conjugated anti-HA (Sigma- 584 583 Aldrich) at 1:1000 dilution, HRP-conjugated anti-mouse IgG (Sigma-Aldrich) at 1:8000 dilution, HRP-conjugated anti-rat IgG (Sigma-Aldrich) at 1:4000 dilution, and HRP-conjugated protein A (Life 585 586 immunoblotting. | Get A Quote |
Some DNA-dependent RNA polymerases (DdRPs) possess RNA-dependent RNA polymerase activity, as was first discovered in the replication of Potato spindle tuber viroid (PSTVd) RNA genome in tomato (Solanum lycopersicum). Recent studies revealed that this activity in bacteria and mammals is important for transcriptional and posttranscriptional regulatory mechanisms. Here, we used PSTVd as a model to uncover auxiliary factors essential for RNA-templated transcription by DdRP PSTVd replication in the nucleoplasm generates (-)-PSTVd intermediates and (+)-PSTVd copies. We found that the Nicotiana benthamiana canonical 9-zinc finger (ZF) Transcription Factor IIIA (TFIIIA-9ZF) as well as its variant TFIIIA-7ZF interac... More