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A novel mechanism of sequestering fibroblast growth factor 2 by glypican in lipid rafts, allowing skeletal muscle differentiation.

Mol. Cell. Biol.. 2010; 
Gutiérrez Jaime,Brandan Enr
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ORF cDNA Clones/MolecularCloud The target sequence for the shRNA against mouse glypican-1 (shGly) (GenScript ORF NM_016696) was as follows: 5⬘-GACCATCCGCCAGCAGA TTATGC-3⬘. Get A Quote

摘要

Heparan sulfate proteoglycans (HSPGs) are critical modulators of growth factor activities. Skeletal muscle differentiation is strongly inhibited by fibroblast growth factor 2 (FGF-2). We have shown that HSPGs present at the plasma membrane are expressed in myoblasts and are downregulated during muscle differentiation. An exception is glypican-1, which is present throughout the myogenic process. Myoblasts that do not express glypican-1 exhibit defective differentiation, with an increase in the receptor binding of FGF-2, concomitant with increased signaling. Glypican-1-deficient myoblasts show decreased expression of myogenin, the master gene that controls myogenesis, myosin, and the myoblast fusion i... More

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