Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | MATERIALS AND METHODS Antibodies The following antibodies against human proteins were pur- chased from the following suppliers: rabbit polyclonal anti- VAMP1 (Synaptic Systems, Göttingen, Germany), mouse monoclonal anti-VAMP2 (R&D systems, Minneapolis, MN, USA), rabbit polyclonal anti-VAMP2 (Abcam, Cambridge, UK), rabbit polyclonal anti-VAMP3 (Abcam), anti-VAMP4 and anti-VAMP8 rabbit polyclonal antibodies (Synaptic Sys- tems), rabbit polyclonal anti-VAMP5 (Biorbyt, Cambridge, UK and Sigma-Aldrich, Madrid, Spain), mouse monoclonal anti- VAMP7 (Covalab, Cambridge, UK), goat anti-HA HRP (Horse- radish peroxidase)-conjugated (GenScript, Piscataway, NJ, USA) and mouse monoclonal anti-β-actin (Abcam). | Get A Quote |
The production and secretion of antibodies by human plasma cells (PCs) are two essential processes of humoral immunity. The secretion process relies on a group of proteins known as soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which are located in the plasma membrane (t-SNAREs) and in the antibody-carrying vesicle membrane (v-SNARE), and mediate the fusion of both membranes. We have previously shown that SNAP23 and STX4 are the t-SNAREs responsible for antibody secretion. Here, using human PCs and antibody-secreting cell lines, we studied and characterized the expression and subcellular distribution of vesicle associated membrane protein (VAMP) isoforms, demonstratin... More