| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Antibody Services | , 2016 Cat# C2987I pRG205MX Addgene 64536 Chemicals, Peptides, and Recombinant Proteins (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911 Methyl methanesulfonate, 99% Sigma-Aldrich Cat# 129925 HU, 98%, powder Sigma-Aldrich Cat# H8627 Zeocin Selection Reagent Thermo Fisher Cat# R25001 a-Factor Mating Pheromone, yeast GenScript Cat# RP01002 Pronase from Streptomyces griseus Sigma-Aldrich Cat# 10165921001 5-Fluoroorotic Acid Monohydrate (FOA, 5-FOA) L-Canavanine sulfate salt, R 99% (TLC), powder US Biological Cat# F5050 Sigma-Aldrich Cat# C9758 Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88803 SsoAdvanced Universal SYBR â Green Supermix Bio-rad Cat# 1725271 Critical Commercial Assays QuikChange II Site-Directed Mutagenesis Kit Agilent Cat# #200523 Experimental Models: Organisms/Strains S...., 2012) and yeast codon optimization was ordered from GenScript. | Get A Quote |
The yeast Mre11-Rad50-Xrs2 (MRX) complex has structural， signaling， and catalytic functions in the response to DNA damage. Xrs2， the eukaryotic-specific component of the complex， is required for nuclear import of Mre11 and Rad50 and to recruit the Tel1 kinase to damage sites. We show that nuclear-localized MR complex (Mre11-NLS) catalyzes homology-dependent repair without Xrs2， but MR cannot activate Tel1， and it fails to tether DSBs， resulting in sensitivity to genotoxins， replisome instability， and increased gross chromosome rearrangements (GCRs). Fusing the Tel1 interaction domain from Xrs2 to Mre11-NLS is sufficient to restore telomere elongation and Tel1 signaling to Xrs2-deficient cells... More
