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Proteomic analysis of Tianzhu White Yak (Bos grunniens) testis at different sexual developmental stages

Anim Sci J.. 2019; 
Ruan CM1,2, Wang J3, Yang YX4, Hu JJ1, Ma YJ5, Zhang Y1, Zhao XX1.
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Protein Tools The subcellular localizations of the 29 identified DEPs were analyzed usingWoLFPSORT (http://www.genscript.com/psort/wolf_psort.html)and ESLpred (http://www.imtech.res.in/raghava/eslpred/). Get A Quote

摘要

To explore the protein expression profiles of white yak (Bos grunniens) testis at different sexual developmental stages. The protein profiles of yak testis were determined using two-dimensional electrophoresis and the expression levels of 298 protein spots were analyzed. Mass spectrometry was performed to identify those significantly differential expressed proteins; Western blotting was used to confirm the results. During the developmental stages, 29 protein spots showed more than twofold differences (p < 0.05) at ≥1 time point and were successfully identified. Two proteins were upregulated with age (category 1), five proteins (17.2%) were downregulated with age (category 2), four proteins were upregulated ... More

关键词

Tianzhu White yak; proteome; sexual maturation; testis; two-dimensional gel electrophoresis