| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | For Western blotting, samples were boiled for 5 min at 95˚C for in loading buffer (50 mM Tris pH 6.8, 40% glycerol, 4% SDS, bromophenol blue) without reducing agent before loading onto a 4–16% PAGE gel (GenScript ExpressPlus™). | Get A Quote |
The group of neurodegenerative diseases, Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA) all exhibit inclusions containing amyloid-type α-synuclein (α-syn) aggregates within degenerating brain cells. α-syn also exists as soluble oligomeric species that are hypothesized to represent intermediates between its native and aggregated states. These oligomers are present in brain extracts from patients suffering from synucleinopathies and hold great potential as biomarkers. Although easily prepared in vitro, oligomers are metastable and dissociate over time, thereby complicating α-syn oligomer research. Using the small amine-reactive cross-linker, formaldehyde (FA), we ... More
