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Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A

J Org Chem.. 2019; 
Liu Y1, Su L1, Fang Q2, Tabudravu J3, Yang X4, Rickaby K2, Trembleau L2, Kyeremeh K5, Deng Z1, Deng H2, Yu Y1.
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摘要

Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis.

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