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Structures of maintenance of carboxysome distribution Walker-box McdA and McdB adaptor homologs

Nucleic Acids Res.. 2019; 
Schumacher MA1, Henderson M1, Zhang H1.
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Bacterial Expression System Artificial genes (codon optimized for E. coli expression) encoding the McdB and McdA homologs from the Cyanothece PCC7424 plasmid (herein referred to as McdB and McdA) were purchased from Genscript and cloned into pET15b between the BamHI and NdeI restriction sites.  Get A Quote

摘要

Carboxysomes, protein-coated organelles in cyanobacteria, are important in global carbon fixation. However, these organelles are present at low copy in each cell and hence must be segregated to ensure transmission from one generation to the next. Recent studies revealed that a DNA partition-like ParA-ParB system mediates carboxysome maintenance, called McdA-McdB. Here, we describe the first McdA and McdB homolog structures. McdA is similar to partition ParA Walker-box proteins, but lacks the P-loop signature lysine involved in ATP binding. Strikingly, a McdA-ATP structure shows that a lysine distant from the P-loop and conserved in McdA homologs, enables ATP-dependent nucleotide sandwich dimer formation. Simila... More

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