| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … The Codon Adaptation index and GC content of the codon-optimized cre gene were<br> calculated using the Rare Codon Analysis Tool from GeneScript (https://www.<b>genscript</b>.<br> com/tools/rare-codon-analysis). The adapted cre gene … | Get A Quote |
The presence of antibiotic resistance genes in genetically modified bacteria raises a regulatory concern in the production of therapeutic proteins and additionally reduces the number of plasmids available for propagation in a cell. Cre recombinase from bacteriophage P1, involved in Cre/loxP mechanism is one of the widely used systems for selectable marker gene removal. We have overexpressed codon-optimized cre gene in pColdIV and pET28a(+) vector systems and purified His6-Cre recombinase by immobilized metal affinity chromatography. N-terminal His6 tagged Cre recombinase obtained was approximately 26 fold purified and promoted the site-specific recombination of two loxP sites of linearized pLox2+ vector allowin... More
